Background:There is an emerging awareness that acutely ill and immobilized neonates are at risk for iatrogenic pressure injury. Therefore, it is crucial for nurses to understand the physiologic indices of pressure injury development and their interventions should be based on evidence-based information to reduce neonatal morbidity and mortality while increasing quality of care in the neonatal care setting.The aim of this study was to evaluate the effectiveness of preventivebundle guidelineson reducing iatrogenic pressure injuries among critically ill neonates.Research design: A quasi-experimental design was used. Settings:This study was conducted at the neonatalintensive care units in Benha Specialized Pediatric Hospital.Sample:A Convenient sample of nurses' (n=50) and critically ill neonates(n=60) were included from the above thementioned setting.Those neonateswere divided equally into two groups(study and control).Tools of data collection:Four tools were used namely; structured interviewing questionnaire format,pressure injury preventive bundle compliance checklist,Braden Q Risk Assessment Scales, andnurses opinion likert scale. Results:High percentage of the neonates (76.7%& 83.3%), in the study group were not at risk of pressure injury in the first and second week of admission respectively;this increased to 90.0%before discharge. While around (40%) of neonates in the control group were at high risk of pressure injury from the first week of admission till before discharge. Moreover. The result revealed that there was a significant improvement in nurses knowledge regarding pressure injuries as well as their compliance with preventive bundle guidelines after bundle implementation.Conclusion:The implementation of preventive bundle guidelines proved to be effective in improving nurses' knowledge and their compliance, with a positive impactontheincidence of pressure injury among critically ill neonates. Recommendation: Preventive bundle guidelines should be implemented in all hospitals caring for children to reduce the harm associated with hospital-acquired pressure injuries.