FORMULATION AND EVALUATION OF IVABRADINE HYDROCHLORIDE LOADED EGG ALBUMIN MICROSPHERES

Abstract

The present work is to formulate and evaluate Ivabradine HCl (IBH) microspheres using egg albumin with the aim to get the best possible drug- polymer ratio giving the sustained drug release. IBH loaded egg albumin microspheres were prepared by heat denaturation technique. Various evaluation parameters were assessed, with a view to obtain sustained release of drug. The prepared IBH microspheres were then subjected to FTIR, SEM, particle size and size distribution, % yield, % drug loading, entrapment efficiency, in vitro dissolution studies, release kinetics and DSC. Different concentration of egg albumin polymer was used to maintain a suitable lag period. The FTIR Spectras revealed that, there was no interaction between the polymer and drug. IBH microspheres were spherical in nature, which was confirmed by SEM. Microspheres with normal frequency distribution were obtained. A maximum of 86.12% drug entrapment efficiency was obtained in the drug loaded microspheres. The in-vitro dissolution data maximum of 73.26% cum. drug release was obtained in the IBH loaded microspheres. The in-vitro performance of IBH microspheres showed that sustained release was dependent upon the polymer concentration. The co-efficient of determination indicated that the release data was best fitted with zero order kinetics. The DSC pattern shows that there was decrease in the crystallinity of the IBH. The present study conclusively demonstrates the feasibility of effectively encapsulating Ivabradine HCl into egg albumin microspheres to form potential sustained release drug delivery system. On comparing the dissolution data of all the formulation, the best release was obtained from AF2 formulation, therefore it can be concluded that among all four drug: polymer ratios, AF2 is the best suitable formulation of IBH microspheres as a sustained drug delivery system.