“IN VITRO STUDIES ON ANTIMICROBIAL AND THROMBOLYTIC ACTIVITY OF SWIETENIA MACROPHYLLA KING”

Abstract

The in vitro techniques & the biological investigations- microbiological and the phytochemical  tests, which  includes  antimicrobial and thrombolytic study  of  the leaves of  Mehagoni  or  Swietenia macrophylla King 3.This plant is used in the traditional medicinal system for cure of a variety of diseases. The leaves of  the  plant were collected locally, Chittagong. The leaves  of  the plants  were extracted by extraction process  using  the ethanol  and acetone  as  solvent. The  antibacterial activity was determined  by  using  the  agar  diffusion  method.  The extract of  the plant showed moderate  activity  against  Staphylococcus aureus ,  Bacillus subtitis, and Pseudomonas aeruginosa . One concentration  of the extracted sample, 30µg/disk was used for  antibacterial activity.  The aqueous  extract  of  the  plant  possess  thrombolytic  activity or not.  An  in vitro  thrombolytic model was used  to check  the clotlysis  effect of aqueous herbal  extracts , along  with streptokinase as a positive  control and water as a negative control. Using  an  in vitro  thrombolytic model the extract– showed  thrombolytic  activity respectively. Through our study it was found that  it possesses thrombolytic properties  that  could  lyses  blood  clots in vitro;  however, in vivo clot dissolving  properties and active components for clotlysis  are  yet  to be discovered.Once found could  be  incorporated as a thrombolytic agent for the improvement of patients  suffering from Atherothrombotic diseases.