PRELIMINARY PHYTO-CHEMICAL INVESTIGATIONS OF METHANOLIC ROOTS EXTRACT OF CLERODENDRUM SERRATUM: ANTIULCER ACTIVITY AND HISTOPATHOLOGICAL STUDY OF STOMACH MUCOSA OF WISTAR RATS IN ETHANOL INDUCED ULCER
Abstract
Different parts of plant contain different constituents, which vary in activity, concentration or purity. The therapeutic potential of Clerodendrum serratum especially antiulcer activity is documented in traditional books and literature. As till date there is no scientific data on antiulcer activity of roots of Clerodendrum serratum. Therefore, the present research work is aimed at evaluating antiulcer potential of the plant data and establish scientific basis for the same. Successive extraction was carried out on the roots of Clerodendrum serratum. Percentage yield of successive extraction were found as 1.65 and 44.27 for petroleum ether and methanol solvent respectively. The phytoconstituents were identified by chemical tests, which showed the presence of various phytoconstituents (carbohydrate, tannin, terpenoid) in methanolic roots extract of Clerodendrum serratum. In ethanol induced model methanolic root extract of Clerodendrum serratum at a dose of (200 mg/kg, p.o) has produced signiï¬cant inhibition of ulcerative lesion by 53.2% as compared to the control group. Rabeprazole significantly decreased number of ulcer, ulcer score and ulcer index as compared to the control value. It showed also significant (p≤0.01) decrease in number of ulcers, ulcer score and ulcer index in ethanol induced ulcer. Ethanol produced reactive species which are responsible for mucosal injury and lipid peroxidation, a free radical mediated process that ultimately destroys lipid membrane. The methanol extract of Clerodendrum serratum roots possess significant antiulcer property in a dose dependent manner by improving gastric mucosal defense mechanism.
References
2. Bhujbal, S.S., Kewatkar, S.M.K., More, L.S., Patil, M.J., 2009. Antioxidant effects of roots of Clerodendrum serratum Linn. a Pharmacognosy Research 5(1), 283-284.
3. Butler, K.L., 2006. Peptic Ulcer Disease and Gastroesophaseal reflux disease. In: Herfindal, E.T., Helms, R., Quam, D.J., Gourley, D.R. (Eds), Text book of therapeutic drugs disease management, Eighth ed. Lipincott Williams and Wilkins, New York, pp.1227-1228 (Chapter 45).
4. Evans, W.C., C.E,Trease., 2002. Pharmacognosy, Fifth ed. Haarcou Brace and Company, pp 336-340.
5. Kalashelavan, V., Franklin, G., Vithalrao, K.P., 2009. Antiulcer activity of methanolic extract Ficus arotina (Moreceae) leaf. American Journal of Pharmacology and Toxicology 4 (3), 89-93.
6. Kenneth, R., Quaid, M., 2000. Drugs used in the treatment gastrointestinal disease. In: Kat Jung, B.G. (Ed), Basic and Clinical Pharmacology, tenth ed. McGraw-Hill, New York, pp. 1009-1011 (chapter 63).
7. Kokate, C.K., Purohit, A.P., Gokhale, S.B., 2002. Pharmacognosy, Nirali prakashan, Pune, p.5.
8. Krishna, V., Naika, H.R., 2008. Plant regeneration from callus culture of Clematis gouriana Roxb.-A Rare Medicinal Plant, Turk Journal Biol 32, 99-103.
9. Mukherjee, P.K., 2008. Quality control Herbal drugs, Business Horizons Pharmaceutical publishers, pp. 426-427.
10. Prasad, V.S., Dhamanigi, S.S., Asad, M., Khare, S., 2008. Antiulcer activity of cod liver oil in rats. Indian J Pharmacol 40 (5) 204-214.
11. Roldao, F.D.E., Witaicenisa, A., Seito, L.N., Hiruma-Lima, C.A., Stasi, L.C., 2008 Evaluation of the antiulcerogenic and analgesic activities of Cordia verbenacea DC. (Boraginaceae). Journal of Ethnopharmacology 119, 94-98.
12. Rueda, R.M., 1993. The genus Clerodendrum (verbenaceae) in Mesoamerica. Annals of the Missouri Botanical Garden 80, 870-890
13. Sairam, K., Goel, R.K., 2002. Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiber officinale. Indian Journal of Pharmacology 37, 100-110.
14. Sandhya, S., Diwakar, M.C., Kumar, K.R., 2010. Evaluation of antiulcer activity of root and leaf extract of Polysciasbalfouriana marginata. J. Chem. Pharm. Res 2,192-195.
15. Sannomiya, M., Fonseca, B.V., Silva, M.A., 2005. Flavonoids and antiulcerogenic ativity from Byrsonima crassa leaves extracts. Journal of Ethnopharmacology 97, 1-6.
16. Shah, R., Nature’s Medicinal plant of Uttaranchal, 2006. Gyanodaya prakhasan, Nainital, pp. 330-331.
17. Shargel, L., Mutnik, A., Souney, H., Swanson, L.N., 2004. Comprehensive pharmacy review, fourth ed. Lippincott William and Wilkins, pp. 948-954.
18. Sharma, SK., Katewa, SS., 2007. Addition to the flora of Rajasthan from southern arravalis, Zoos print journal 10, 2867.
19. Stanley, R., Kumar, V., Cotran, R., 1992. Gastrointestinal tract. Basic Pathology, fifth ed. Prism Book, Bangalore, p. 473 (Chapter 15).
20. Victoria, U.C., Michael, U.C., Johmmy, M.O., 2010. Evaluation of antiulcer activity of Olax subscorpioida Olive roots in rats. Asia Pacific Journal Tropical medicine, 13-16.
21. Wahida, B., Abderrahman, B., Chouchane, N., 2007. Antiulcerogenic activity of Zizyphus lotus (L.) extracts. Journal of Ethnopharmacology 112, 228-231.
