In vitro antiplasmodial activity of Kani herb Alstonia scholaris against Plasmodium falciparum

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C. C. S. P. kumar J. M. beula, N. C. L. N. Jeyaraj, S. Ravikumar, “In vitro antiplasmodial activity of Kani herb Alstonia scholaris against Plasmodium falciparum”, ijmhs, vol. 5, no. 4, pp. 166–169, Aug. 2015.
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Abstract

Background: The Alstonia scholaris plant grows throughout India, in deciduous and evergreen forests and also in plains. The plant is known to possess a lot of medicinal properties in folk medicine by the Kani tribals in Kanyakumari district. A. scholaris bark has been as one of the ingredient used in the marketed Ayurveda preparation Ayush-64, NRDC, India. The milky juice of the plant is applied on wounds, ulcers and rheumatic pains. Tincture of the bark and juice of the leaves act as powerful galactogogue in certain cases. The drug is also used in case of snake bite. Objectives: The present study has been made an attempt to find out the antiplasmodial activity of different plant parts (Leaf, root, bark, stem bark and fruit) of A. scholaris against the most prevalent Indian malarial Parasite P. falciparum. Methods: Different plant arts Viz., Leaf, stem, bark, root and fruit of A. scholaris plant was collected from SouthWest coast of India, Kanyakumari, Tamil Nadu during monsoon and non-monsoon months. The plant parts were cut into pieces and kept for shade drying. Moisture free samples were subjected for percolation by soaking it in ethanol: water mixture (3:1 ratio). Different concentrations of filter-sterilized crude extract from plants (100, 50, 25, 12.5, 6.25 and 3.125 µg. ml−1) was incorporated into 96-well tissue culture plate containing 200 μl of P. falciparum culture with fresh red blood cells diluted to 2% haematocrit. Results: Among the plant parts tested against P. falciparum, the bark and leaf extract of A. scholaris collected during non-monsoon season exhibited IC50<3.125 µg. ml-1; followed by the stem extract (IC50 3.125 µg. ml-1). Conclusion: It can be concluded from the present study that the bark and leaf extracts of A. scholaris potentiate the antiplasmodial activity against P. falciparum.

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