Effective anaerobic culture method for identifying the organisms in deep dentinal caries and testing the antimicrobial efficacy of the pulp capping agents- comparison of 2 techniques

  • Soumya Makarla
  • Reshma Venugopal
  • Radhika Manoj Bavle
  • Arul Selvan K
  • Sudhakara Muniswamappa
  • Prashanth Ramachandra
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Soumya Makarla, Reshma Venugopal, Radhika Manoj Bavle, Arul Selvan K, Sudhakara Muniswamappa, and Prashanth Ramachandra, “Effective anaerobic culture method for identifying the organisms in deep dentinal caries and testing the antimicrobial efficacy of the pulp capping agents- comparison of 2 techniques”, ijmhs, vol. 11, no. 02 (Mar - April), pp. 1615–1621, Mar. 2021.
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Abstract

Background: Effective pulp capping cements with good antimicrobial properties is a requirement to prevent propagation of deep dentinal caries to healthy pulp. Previously, the studies on antimicrobial efficacy were performed using commercially available strains. The present study was carried out using the samples collected directly from deep dentinal caries. Two different methods direct contact test (DCT) and agar diffusion method (AGM) of anaerobic incubation were assessed to predict the antimicrobial efficacy of the dental cements. Aims: To test, compare and gauge 2 anaerobic methods- DCT and ADM to detect the antimicrobial efficacy of the cements. Materials and Methods: Carious material (n=25) from deep dentinal caries. Glass ionomer (GIC Fuji II), CaOH2 in form of Dycal, mineral trioxide aggregate (MTA) and Zinc oxide eugenol (ZnOE) were tested for antimicrobial efficacy using DCT and ADM. Results and statistical analysis: The anaerobes could be grown with both the techniques (100%). Contamination was detected in 12% of cases on DCT; MTA plates were contaminated the most. Lack of diffusion of cements across the agar was seen in 12% of cases of ADM. Both the techniques showed ZnOE to be more effective followed by MTA, Dycal and GIC with a p value of >0.001 and was statistically significant. Conclusion: DCT is an extensive procedure and selective colonies could be grown. ADM is easy to perform and allowed the growth of mixed culture. The disadvantages of DCT were contamination and prolonged procedure, whereas; of ADM were poor diffusion of the cements across the agar.

Keywords: Anaerobic culture, Mixed microflora, Deep dental caries, Pulp capping agents, Anaerobic culture technique, Direct contact test, Agar diffusion method

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