EVALUATION OF THE EXPRESSION OF LUXS GENE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS FROM BLOODSTREAM INFECTIONS

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T. T. L. I. B. U. Riekstina U., Zilevica A., “EVALUATION OF THE EXPRESSION OF LUXS GENE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS FROM BLOODSTREAM INFECTIONS”, ijmhs, vol. 2, no. 4, Oct. 2013.
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Abstract

Staphylococcus epidermidis is a common, nosocomial bacteria causing bloodstream infections. There is an urgent need to develop techniques which assist for differentiating between invasive and contaminating strains. Phenol-soluble modulin (PSM) peptides important for biofilm formation of S.epidermidis are regulated by the luxS/Autoinducer-2. To evaluate the expression of luxS gene for diagnostic purpose, we analysed changes in gene expression in vitro in S.epidermidis specimens isolated from bloodstream infections (n = 43) and in those from a control group (n = 35) produced by quantitative PCR. The mean expression of luxS gene was not significantly different between the clinical and control groups. We also studied correlation of the expression of luxS gene with previously described icaADBC operon. The presence of icaADBC operon regulating polysaccharide intercellular adhesin (PIA) production was detected by amplification of icaA gene fragment in all isolates. S.epidermidis isolates from patients with bloodstream infections were not different in presence of icaA gene from the nasal flora of healthy volunteers. In the clinical group, the mean expression of luxS gene was significantly higher (p = 0,0026) in isolates positive for icaA (icaA+) then in isolates negative for icaA (icaA-) gene (21,3% and 8,3%, respectively). The results of our study show that the capacity to form biofilm in vivo is strongly influenced by many environmental factors independent of luxS expression and the icaADBC products.

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