EVALUATION OF THE EXPRESSION OF LUXS GENE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS FROM BLOODSTREAM INFECTIONS

Cite this:
[1]
T. T. L. I. B. U. Riekstina U., Zilevica A., “EVALUATION OF THE EXPRESSION OF LUXS GENE IN CLINICAL ISOLATES OF STAPHYLOCOCCUS EPIDERMIDIS FROM BLOODSTREAM INFECTIONS”, ijmhs, vol. 2, no. 4, Oct. 2013.
© 2022 Interactive Protocols
RIGHTS & PERMISSIONS
Article Views
160
Altmetric
1
Citations
-
LEARN ABOUT THESE METRICS
   PDF  

Abstract

Staphylococcus epidermidis is a common, nosocomial bacteria causing bloodstream infections. There is an urgent need to develop techniques which assist for differentiating between invasive and contaminating strains. Phenol-soluble modulin (PSM) peptides important for biofilm formation of S.epidermidis are regulated by the luxS/Autoinducer-2. To evaluate the expression of luxS gene for diagnostic purpose, we analysed changes in gene expression in vitro in S.epidermidis specimens isolated from bloodstream infections (n = 43) and in those from a control group (n = 35) produced by quantitative PCR. The mean expression of luxS gene was not significantly different between the clinical and control groups. We also studied correlation of the expression of luxS gene with previously described icaADBC operon. The presence of icaADBC operon regulating polysaccharide intercellular adhesin (PIA) production was detected by amplification of icaA gene fragment in all isolates. S.epidermidis isolates from patients with bloodstream infections were not different in presence of icaA gene from the nasal flora of healthy volunteers. In the clinical group, the mean expression of luxS gene was significantly higher (p = 0,0026) in isolates positive for icaA (icaA+) then in isolates negative for icaA (icaA-) gene (21,3% and 8,3%, respectively). The results of our study show that the capacity to form biofilm in vivo is strongly influenced by many environmental factors independent of luxS expression and the icaADBC products.

KEYWORDS:

 Special Issue

Article Metrics Graph

Chart Graph | Range Graph

Content

Section

Source

License

All paper submissions must carry the following duly signed by all the authors:
“I certify that I have participated sufficiently in the conception and design of this work and the analysis of the data (wherever applicable), as well as the writing of the manuscript, to take public responsibility for it. I believe the manuscript represents valid work. I have reviewed the final version of the manuscript and approve it for publication. Neither has the manuscript nor one with substantially similar content under my authorship been published nor is being considered for publication elsewhere, except as described in an attachment. Furthermore I attest that I shall produce the data upon which the manuscript is based for examination by the editors or their assignees, if requested.”