Innovative Journal of Medical and Health Sciences

Priya Dhote*, Alka Chaturvedi

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AERVA LANATA [L.] A.L. JUSS. is an important medicinal plant known for its prolonged hypertensive and hypoglycemic activity. In the present study callusing was achieved in Aerva lanata plant. Response of callus of Aerva lanata was observed on MS medium supplemented with BAP, NAA and IAA in various combinations.

Ranjit Kumar *, Shivshanker Kumar, Mohd Ali, Arun Kumar, A. Nath, Kapil Lawrence , J.K. Singh

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Epinephrine is a hormone and neurotransmitter. When produced in the body it increases heart rate, contracts blood vessels and dilates air passages and participates in the "fight or flight" response of the sympathetic nervous system. During stress concentration of epinephrine were increased many times. In present study epinephrine were administered to mice @ 200nl/k.g.bw to induce stress in animal, which were dissected after 2 and 4 weeks. SGOT and Urea level in serum were increased in epinephrine administered group. Liver and kidney tissue were fixed for light microscopy and serum were collected for SGOT and urea assay. While after administration of epinephrine frequent vacuolisation were observed. Elongated nucleus was also observed in hepatic cells. Degenerated hepatic vein were also observed. While in kidney dilated bowmen’s capsule were observed. Frequent vacuolization were also observed in cortex region. Endothelial cells of PCT were observed in degenerated condition. Thus it is concluded from entire study that stress causes degeneration in hepatic cells, central vein, glomerulus, bowmens capsule, PCT and DCT which finally leading to both hepato-toxicity and nephro-toxicity.

Tracevska T*, Liduma1 I., Bers U, Riekstina U., Zilevica A.

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Staphylococcus epidermidis is a common, nosocomial bacteria causing bloodstream infections. There is an urgent need to develop techniques which assist for differentiating between invasive and contaminating strains. Phenol-soluble modulin (PSM) peptides important for biofilm formation of S.epidermidis are regulated by the luxS/Autoinducer-2. To evaluate the expression of luxS gene for diagnostic purpose, we analysed changes in gene expression in vitro in S.epidermidis specimens isolated from bloodstream infections (n = 43) and in those from a control group (n = 35) produced by quantitative PCR. The mean expression of luxS gene was not significantly different between the clinical and control groups. We also studied correlation of the expression of luxS gene with previously described icaADBC operon. The presence of icaADBC operon regulating polysaccharide intercellular adhesin (PIA) production was detected by amplification of icaA gene fragment in all isolates. S.epidermidis isolates from patients with bloodstream infections were not different in presence of icaA gene from the nasal flora of healthy volunteers. In the clinical group, the mean expression of luxS gene was significantly higher (p = 0,0026) in isolates positive for icaA (icaA+) then in isolates negative for icaA (icaA-) gene (21,3% and 8,3%, respectively). The results of our study show that the capacity to form biofilm in vivo is strongly influenced by many environmental factors independent of luxS expression and the icaADBC products.