COMPARISON OF HIV-1 DNA PCR RESULTS OBTAINED FROM FRESH AND STORED DRIED BLOOD SPOTS OF HIV-1 EXPOSED INFANTS

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S. M. J. S. P. G. A. Ravi Rajkumar, Durairaj Anitha, Kamala Mary, “COMPARISON OF HIV-1 DNA PCR RESULTS OBTAINED FROM FRESH AND STORED DRIED BLOOD SPOTS OF HIV-1 EXPOSED INFANTS”, ijmhs, vol. 3, no. 1, Oct. 2013.
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Abstract

Purpose of the study: We compared the results of fresh dried blood spot (DBS) collected on filter paper (Whatman 903) and stored at room temperature for six months and one year. Basic Procedures:  HIV testing was done using Roche Amplicor HIV-1 DNA Kit. The Optical Density (OD) values of fresh samples, stored samples at six months and one year were compared.  Main Findings: Thirty-two of 400 DBS tested HIV positive. There was no significant difference between OD values of fresh DBS and stored DBS at six months and one year.  Principal Conclusions: HIV-1 DNA was stable for one year on filter paper at room temperature

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