##submission.abstract##
The use of medicinal plants has been encouraged by the World Health Organization because of its traditional use in crops and the benefits conferred its users. The development of antimicrobial drugs is important for the development of new sources for the therapeutic treatment of infectious diseases, since the characteristic of bacteria have become resistant to the action of antibiotics. The aim of this study was to evaluate the influence of an alcoholic extract of the bark of Punica granatum fruit as its possible genotoxicity, cytotoxicity and mutagenic action against the bacterium Staphylococcus aureus. To evaluate cytotoxicity, we employed the method of diffusion of nutrient agar disk, assessing the bacterial growth compared to the presence of impregnated disks of different volumes of extract and antibiotics. For the determination of genotoxicity and mutagenicity, plus extract were employed as genotoxic and mutagenic agents hydrogen peroxide and chloride stannous. The results showed that the extract has antibiotics like gentamicin, chloramphenicol, stannous chloride and hydrogen peroxide effect. The results suggest that the extract of Punica granatum probably has cytotoxic, genotoxic and mutagenic action, but there is a need for more specific analyzes to further elucidate the presence of the latter characteristic.